Methods and compositions for biomarkers of fatigue

摘要

The present invention provides methods and compositions for identifying fatigue, disease states associated with fatigue, recovery from fatigue and/or physical performance capability in a subject.

主权项

1. A method of guiding a human subject's treatment of fatigue, comprising:
a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO: 1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to an equation selected from the group consisting of: 1) PPGKPQQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:)/total protein (μg), 2) GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2)/total protein (μg), 3) SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3)/total protein (μg), 4) ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ; SEQ ID NO:1]+[GNPQGPSPQOGGNKPQGPPPPPGKPQ; SEQ ID NO:2]/total protein (μg) 5) ([PPGKPQGPPPQGGNQPQQGPPPPPGKPQ; SEQ ID NO: 1]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ; SEQ ID NO:3])/total protein (μg) 6) ([GNPQGPSPQGGNKPQGPPPPPGKPQ; SEQ ID NO:2]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ; SEQ ID NO:3])/total protein (μg) and 7) ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ; SEQ ID NO:1]+[GNPQGPSPQGGNKPQGPPPPPGKPQ; SEQ ID NO:2]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ; SEQ ID NO:3])/total protein (μg); c) treating the subject for fatigue; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation(s) selected in (b); and f) guiding the subject's treatment of fatigue using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the ratio as calculated in (b) leads to a subsequent enhancement of the treatment for fatigue, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change or a subsequent reduction of the treatment for fatigue.