Method of identifying fetal antigens or cell surface markers using phage display technology

摘要

The present application relates to methods for identification of foetal cells and generation and isolation of binding members recognizing foetal cells. The methods of the invention may further be used for other purposes relating to characterization of biological samples and biological antigens. The methods are characterized by the applicability in situations where the interesting objects are present in a limited amount, or where the interesting objects are intermixed with other material, thus the methods are suitable for use in situations where the ratio of the interesting material compared to other material is low.

主权项

1. A method of isolating binding members recognizing an antigen of a target cell of limited availability in a heterogeneous mixture of target cells and non-target cells comprising the steps:
a. identifying the target cells, b. contacting said target cells with a binding member generating system that allows linkage between the genotype and the phenotype of the binding member and is selected from the group consisting of: ribosome display, DNA display, yeast display, bacterial display, retroviral display and phage display, c. washing said target cells to avoid drying, d. protecting binding members associated with said target cells with a shield, wherein the size of said shield is 60-200 μm in width and placed close to the target cells, e. minimizing the number of binding members isolated recognizing non-target cells by subjecting the non-target cells to UV light capable of introducing cross-links in the genetic material of the binding members, whereby the genetic information is rendered non-replicable, said shield from step d protecting binding members recognizing a target cell against said UV light, and f. isolating binding member(s) recognizing the antigen of the target cell.