Microfluidic devices and method for their use

摘要

Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

主权项

1. A microfluidic device comprising:
a first plurality of fluid channels, wherein one of M different primers can be introduced into each of the first plurality of fluid channels; a second plurality of fluid channels, wherein one or more of N different samples can be introduced into each of the second plurality of fluid channels; a plurality of valves that control fluid flow within the first and the second plurality of fluid channels, wherein at least some of the first plurality of fluid channels and the second plurality of fluid channels intersect to form an array reaction sites; and a thermal cycler comprising:
a plurality of guard channels disposed over or under the reaction sites;a source comprising a hot liquid and a cold liquid that is colder than the hot liquid; anda pump configured to perform thermal cycling by alternately flowing the hot liquid and the cold liquid into the guard channels.