Signal sequence-independent pIX phage display

摘要

The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pIX. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid that contains a nucleic acid encoding the fusion protein of the invention and a helper phage.

主权项

1. A phage genome or a phagemid comprising a nucleic acid encoding a fusion protein comprising the filamentous phage minor coat protein pIX fused to an exogenous peptide, wherein the fusion protein does not comprise an N-terminal signal sequence,
wherein the filamentous phage minor coat protein pIX comprises a sequence selected from the group consisting of position 1-32, 2-32, 3-32, 4-32 and 5-32 of SEQ ID NO: 1 (MSVLVYSFASFVLGWCLRSGITYFTRLMETSS), wherein the exogenous peptide of the fusion protein is fused directly to the N-terminus of the pIX sequence.