| 摘要 |
Disclosed is a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene, wherein said pyripyropene biosynthetic gene cluster comprises the nucleotide sequence selected from the nucleotide sequences in (I) to (IV) below: (I) a nucleotide sequence from 2911 to 27797 in SEQ ID NO:266; (II) a nucleotide sequence which is capable of hybridizing with every nucleotide in the full length complementary sequence from 2911 to 27797 in SEQ ID NO:266 under stringent conditions comprising washing with 2 × saline-sodium citrate (SSC) buffer and 0.5% Sodium dodecyl sulfate (SDS) at 60oC for 20 minutes, and then washing with 0.2 × SSC and 0.1 % SDS at 60oC for 15 minutes, and which encodes a protein substantially equivalent to a protein encoded by the nucleotide sequence from 2911 to 27797 in SEQ ID NO:266; (III) a nucleotide sequence of a polynucleotide of the nucleotide sequence from 2911 to 27797 in SEQ ID NO:266 in which one to four nucleotides are deleted, substituted, inserted or added, and which encodes a protein substantially equivalent to a protein encoded by the nucleotide sequence from 2911 to 27797 in SEQ ID NO:266; and (IV) a nucleotide sequence which has at least 90% identity to a polynucleotide of the nucleotide sequence from 2911 to 27797 in SEQ ID NO:266, and which encodes a protein substantially equivalent to a protein encoded by the nucleotide sequence from 2911 to 27797 in SEQ ID NO:266. Further disclosed is a nucleic acid construct comprises a pyripyropene biosynthetic gene cluster and a marker gene, wherein said pyripyropene biosynthetic gene cluster comprises a nucleotide sequence from 1 to 25000 of a nucleotide sequence shown in SEQ ID NO: 266. |
