发明名称 |
METHOD FOR INTRODUCING EXOGENOUS DNA BY OVERCOMING RESTRICTION MODIFICATION BARRIER OF TARGET BACTERIUM |
摘要 |
The present invention discloses a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method provided in the present invention comprises the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A; 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule; 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium. The experiments of the invention have demonstrated that the invention has a high transformation efficiency compared to prior methods for enabling genetic manipulation by overcoming the restriction modification barrier of the bacterium. |
申请公布号 |
EP2829599(A1) |
申请公布日期 |
2015.01.28 |
申请号 |
EP20130764964 |
申请日期 |
2013.02.21 |
申请人 |
INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES |
发明人 |
WEN, TINGYI;ZHANG, GUOQIANG;DENG, AIHUA |
分类号 |
C12N1/21;C12N1/20;C12N15/24;C12R1/01;C12R1/07;C12R1/085;C12R1/19 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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