POLYPEPTIDE

摘要

FIELD: chemistry.SUBSTANCE: invention relates to field of biochemistry, in particular to polypeptide, possessing amylase activity, derived from parent polypeptide, which represents non-maltogenic exoamylase of wild type, which has at least 90% identity SEQ ID NO: 13. Said polypeptide contains amino acid substitution in position 307 for lysine (K) or arginine (R), in accordance with numeration of positions of Pseudomonas saccharophilia exoamylase sequence, represented in SEQ ID NO: 1. Polypeptide is applied as food or forage additive, in method of starch processing to obtain starch-containing food or forage product, in particular bakery product.EFFECT: invention makes it possible to increase thermal stability and exospecificity of non-maltogenic exoamylase in comparison with wild type polypeptide.62 cl, 14 dwg, 25 tbl, 37 ex