发明名称 |
DNA-bibliotek og fremgangsmåde til fremstilling heraf samt fremgangsmåde og anordning til detektering af SNP |
摘要 |
A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 451; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library. |
申请公布号 |
DK2631336(T3) |
申请公布日期 |
2015.01.26 |
申请号 |
DK20110843141T |
申请日期 |
2011.09.21 |
申请人 |
BGI TECH SOLUTIONS CO., LTD. |
发明人 |
DU, YE;ZHAO, MEIRU;CHEN, YING;WU, JINGHUA;TIAN, GENG;WANG, JUN |
分类号 |
C40B50/06;C12N15/11;C12Q1/68;C40B20/04;C40B40/08 |
主分类号 |
C40B50/06 |
代理机构 |
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代理人 |
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