发明名称 |
SELECTIVE NUCLEIC ACID AMPLIFICATION FROM NUCLEIC ACID POOLS |
摘要 |
Provided herein are nucleic acids and methods for selectively amplifying in parallel tens of thousands of high quality oligonucleotides without common sequences. The resultant oligonucleotides can be used for a variety of purposes and applications including but not limited to DNA nano structure synthesis. |
申请公布号 |
US2015024972(A1) |
申请公布日期 |
2015.01.22 |
申请号 |
US201314376241 |
申请日期 |
2013.02.08 |
申请人 |
President and Fellows of Harvard College |
发明人 |
Schmidt Thorsten Lars;Theilmann Mark;Shih William M. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A single-stranded nucleic acid comprising
a target sequence, a 5′ end having a first sequence, and a 3′ end having a second sequence such that, when the 5′ end and the 3′ end are juxtaposed, the first sequence and the second sequence form a subpool-specific region, wherein the subpool-specific region comprises a subpool-specific sequence and an endonuclease site and is flanked on each end by a nicking site. |
地址 |
Cambridge MA US |