METHOD FOR DETERMINING THE GLYCOSYLATION OF AN ANTIBODY

摘要

The invention relates to a method for detecting the binding of an antibody to an Fc receptor present on the surface of a cell as well as to a method for determining the level of glycosylation of an antibody. The invention also relates to a reagent kit for carrying out these methods.

主权项

1. An in vitro method for determining the binding of an antibody to an Fc receptor expressed in cell membranes or intact cells present in a measurement medium, comprising the following steps:
(i) direct or indirect labeling of said Fc receptor with the first member of a pair of FRET partners, or else introducing, into the medium, cell membranes or intact cells whose Fc receptors were labeled beforehand directly with the first member of a pair of FRET partners; (ii) adding said antibody, labeled directly or indirectly with the second member of said pair of FRET partners, to the measurement medium; (iii) measuring the FRET signal, the existence of a FRET signal being representative of the binding of the antibody to the Fc receptor.