T1R Hetero-Oligomeric Taste Receptors

摘要

Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for identifying putative taste modulating compounds using such hetero-oligomeric complexes also described, as is a novel surface expression facilitating peptide useful for targeting integral plasma membrane proteins to the surface of a cell.

主权项

1. A method of screening for compounds that modulate sweet taste signaling comprising:
(i) contacting a cell co-expressing at least two T1R receptors or polypeptides on its surface with a putative taste modulating compound; and (ii) measuring activity from the T1R receptors or polypeptides expressed on the cell surface wherein the expressed T1R receptors or polypeptides act as a hetero-oligomeric complex.