METHOD AND KIT FOR MEASURING INTERACTION BETWEEN MOLECULES

摘要

The present invention relates to methods of measuring interaction between a first and a second molecule, for example a protein and an antibody, by conjugation of one of these molecules with nanoparticles, and measuring the interaction between the first and second molecule via changes in the optical properties of the nanoparticles. The present invention further relates to methods of coating nanoparticles.

主权项

1. A method of determining an interaction between a first and a second molecule comprising:
a) providing nanorods comprising one or more metals and having an aspect ratio between 1.5 and 5; b) providing said nanorods with one or more functional groups, or coupling said first molecule to a molecule comprising a metal binding functionality; c) conjugating said first molecule to said nanorods, whereby the amount of said first molecule attached to said nanorods is between 30% and 70% of the amount required for full coverage of said nanorods with said first molecule; d) incubating said nanorods with said second molecule; e) monitoring step d) by illuminating said nanorods with at least one excitation light source and monitoring one or more optical properties of said nanorods; and f) detecting a change of one or more optical properties of said nanorods wherein said change is a result of the presence of an interaction between said first molecule and said second molecule.