SIMULTANEOUS, INTEGRATED SELECTION AND EVOLUTION OF ANTIBODY/PROTEIN PERFORMANCE AND EXPRESSION IN PRODUCTION HOSTS

摘要

The present disclosure provides methods of integrating therapeutic protein and antibody generation and/or selection, evolution and expression in a eukaryotic host for manufacturing in a single system. Therapeutic proteins, including antibodies, are generated, optimized and manufactured in the same eukaryotic host system. The disclosed system of Comprehensive Integrated Antibody Optimization (CIAO!™) allows for simultaneous evolution of protein performance and expression optimization.

主权项

1. The method of claim 49, wherein step a comprises steps of:
a1. generating an anti-antigen antibody library in the mammalian cell production host with antibody cell surface display; a2. screening the library for the at least one predetermined property, characteristic or activity; and wherein the template antibody is selected from the library.