METHOD OF IDENTIFICATION OF SPORE-FORMING Bacillus spp. BY DIRECT In-situ ANALYSIS OF MALDI-TOF MASS SPECTROMETRY, AND ANALYSIS SYSTEM

摘要

A method of the identification of Bacillus species by direct in-situ analysis of MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectometry) in which spore-forming bacteria are applied intact without any pretreatment, and an analysis system of distinctive biomarkers which allow Bacillus spores to be distinguished. Rapid and accurate detection and identification of Bacillus species can be achieved by the method and analysis system.

主权项

1. A method of identifying a Bacillus species using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS), comprising:
directly spotting a sample containing an intact spore-forming Bacillus bacterium onto a MALDI target plate without conducting any pretreatment thereto; and performing MALDI-TOF mass spectroscopy to acquire spectral data of the sample; and analyzing the spectral data with reference to a reference data (m/z) to identify the sample, said reference data being established for biomarker peaks distinctive for known Bacillus species.