ENHANCED DEPOSITION OF CHROMOGENS UTILIZING PYRIMIDINE ANALOGS

摘要

This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formula;;where R1, R2, R3, and R4 are independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; R1 and/or R3 can be bound to R2 to form a fused, aromatic ring system; R5 is selected from a heteroatom-containing moiety; A is selected from, a carbon atom, a heteroatom, other than sulfur, and any combination thereof; n is 1-5, an enzyme, a specific binding moiety and a detectable moiety.

主权项

1. A composition for detecting a target in a sample by proximally depositing a marker, comprising:
a pyrimidine and/or pyridine deposition enhancer having a formulawherein R1, R2, R3, and R4 are independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; R1 and/or R3 can be bound to R2 to form a fused, aromatic ring system; R5 is a heteroatom-containing moiety; A is carbon, a heteroatom other than sulfur, and any combination thereof; and n is 1-5;
a second deposition enhancer; and an enzyme substrate.