METHODS FOR IDENTIFYING EUBACTERIA

摘要

This invention relates, e.g., to methods for detecting a eubacterium, determining if the eubacterium is Gram-positive or Gram-negative, and determining the species of the eubacterium in a sample.

主权项

1. A set of oligonucleotides for distinguishing Gram-positive eubacteria from Gram-negative eubacteria, wherein
(a) a first oligonucleotide, which is specific for Gram-positive eubacteria, consists of the sequence TGGTGCATGGTTGT (SEQ ID NO:1);
or a variant thereof in which 1 or 2 of the residues are substituted with other nucleotides, provided that the G and T residues that are indicated with bold underlining are not altered;or a variant of the oligonucleotide consisting of SEQ ID NO:1 or of the substituted variant, which has up to 5 additional nucleotides at its 5′ end from the Propionibacter rRNA gene sequence shown in Table 7 and/or up to 13 additional nucleotides at its 3′ end from the Propionibacter rRNA gene sequence shown in Table 7; and (b) a second oligonucleotide, which is specific for Gram-negative eubacteria, consists of the sequence TGCTGCATGGCTGT (SEQ ID NO:2);
or a variant thereof in which 1 or 2 of the residues are substituted with other nucleotides, provided that the two C residues that are indicated with bold underlining are not altered;or a variant of the oligonucleotide consisting of SEQ ID NO:2 or of the substituted variant which has up to 4 additional nucleotides at its 5′ end from the Acinetobacter rRNA gene sequence shown in Table 7 and/or up to 13 additional nucleotides at its 3′ end from the Acinetobacter rRNA gene sequence shown in Table 7.