DETECTION AND QUANTIFICATION OF ANALYTES BASED ON SIGNAL INDUCED BY ALKALINE PHOSPHATE

摘要

A general methodology for highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method and sensors take advantage of the ability of alkaline phosphatase (ALP) to convert glucose-1-phosphate to glucose, and the ability of PGMs to detect the generated glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents, for example to diagnose disease, are also provided.

主权项

1. A method for detecting a target enzyme activity, comprising:
contacting a test sample with one or more starting products, wherein at least one of the starting products can be converted to glucose-1-phosphate by a target enzyme, comprises an enzyme involved in the production of glucose-1-phosphate, or combinations thereof; allowing the target enzyme to convert the at least one starting product to glucose-1-phosphate; contacting the glucose-1-phosphate with alkaline phosphatase (ALP); allowing the ALP to convert the glucose-1-phosphate to glucose; detecting the glucose with a personal glucose meter (PGM); and determining the target enzyme activity by correlating the glucose detected.