DETECTING AND QUANTIFYING CRYPTIC HIV REPLICATION

摘要

The present invention relates to a novel method for detecting efficient cryptic HIV replication in a patient who receives a suppressive antiviral therapy followed by administration of the HIV integrase inhibitor in an effective amount for intensifying the suppressive antiviral therapy, and has undetectable plasma viremia prior to the administration of the HIV integrase inhibitor. The method comprises making a pre-intensification measurement and one or more post-intensification measurements of the concentration of an episomal artifact in samples from the patient, and computing a pre-intensification HIV infection success ratio (R). A pre-intensification HIV infection success ratio (R) sufficiently close to 1 indicates that the patient has the efficient cryptic HIV replication. The method may further comprise quantifying the efficient cryptic HIV replication.

主权项

1. A method for detecting efficient cryptic HIV replication in a patient, wherein the patient receives a suppressive antiviral therapy not containing an HIV integrase inhibitor followed by administration of the HIV integrase inhibitor in an effective amount for intensifying the suppressive antiviral therapy, and wherein the patient has undetectable plasma viremia prior to the administration of the HIV integrase inhibitor, comprising:
(a) making a pre-intensification measurement of the concentration of an episomal artifact in a pre-intensification sample from the patient; (b) making one or more post-intensification measurements of the concentration of the episomal artifact in one or more post-intensification samples from the patient; and (c) computing, on at least one processor, a pre-intensification HIV infection success ratio (R) based on the pre-intensification measurement and the one or more post-intensification measurements, wherein a pre-intensification HIV infection success ratio (R) sufficiently close to 1 indicates that the patient has the efficient cryptic HIV replication.