GENE TARGETING VECTOR, METHOD FOR MANUFACTURING SAME, AND METHOD FOR USING SAME

摘要

Provided is a gene targeting vector capable of highly efficient gene targeting. A gene targeting vector in which a DNA sequence allowing for bicistronic expression is present 5' upstream of a selection marker. A method for producing a gene targeting vector, comprising linking a DNA fragment homologous to a 5' upstream region of a target site, a selection marker having a DNA sequence allowing for bicistronic expression present 5' upstream thereof, and a DNA fragment homologous to a 3' downstream region of the target site.