FLAVIN-BINDING GLUCOSE DEHYDROGENASE, METHOD FOR MANUFACTURING FLAVIN-BINDING GLUCOSE DEHYDROGENASE, AND GLUCOSE MEASUREMENT METHOD USING SAME

摘要

Provided is a flavin-binding glucose dehydrogenase (FAD-GDH), which in addition to having high substrate specificity and adequate heat stability, is suitable for efficient production preferably using E. coli, yeast or molds and the like as host cells. This FAD-GDH has amino acid substitutions at positions equivalent to one or more locations of the group consisting of position 213, position 368 and position 526 in the amino acid sequence described in SEQ ID NO: 8. The FAD-GDH is acquired from a culture by inserting a gene encoding this FAD-GDH into host cells such as E. coli. A preferable example of the FAD-GDH of the present invention is FAD-GDH, in which a signal peptide region present in an N-terminal region has been deleted from the amino acid sequence of Mucor-derived FAD-GDH, and which has the aforementioned amino acid substitutions. FAD-GDH can be efficiently produced that has high substrate specificity and superior heat stability and can be preferably used in clinical diagnosis and the like.