发明名称 |
NON-HUMAN ANIMALS EXPRESSING PH-SENSITIVE IMMUNOGLOBULIN SEQUENCES |
摘要 |
Genetically modified non-human animals are provided that express an immunoglobulin variable domain that comprises at least one histidine, wherein the at least one histidine is encoded by a substitution of a non-histidine codon in the germline of the animal with a hisidine codon, or the insertion of a histidine codon in a germline immunoglobulin nucleic acid sequence. Immunoglobulin genes comprising histidines in one or more CDRs, in an N-terminal region, and or in a loop 4 region are also provided. Immunoglobulin variable domains comprising one or more histidines (e.g., histidine clusters) substituted for non-antigen-binding non-histidine residues. Non-human animals that are progeny of animals comprising modified heavy chain variable loci (V, D, J segments), modified light chain variable loci (V, J segments), and rearranged germline light chain genes (VJ sequences) are also provided. Non-human animals that make immunoglobulin domains that bind antigens in a pH-sensitive manner are provided. |
申请公布号 |
EP2825037(A1) |
申请公布日期 |
2015.01.21 |
申请号 |
EP20130714127 |
申请日期 |
2013.03.15 |
申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
MCWHIRTER, JOHN;MACDONALD, LYNN;MARTIN, JOEL, H.;MURPHY, ANDREW, J. |
分类号 |
A01K67/027;C07K16/00;C12N15/85 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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