Primers for detecting plasmodium

摘要

The present invention provides an easy and rapid method for detecting/identifying the presence or absence of specific Plasmodium parasites and four species of malaria parasites in a human specimen, an anti-malaria measure support system, and a malaria infection-prevention/treatment system, which can contribute to practical diagnosis in a malaria endemic area. According to the present invention, using a genus-specific primer set that can detect four Plasmodium parasites that infect humans at a time, and the primer sets each specific to each of four species of Plasmodium parasites (P. falciparum, P. vivax, P. malariae, and P. ovale), the presence or absence of infection with these parasites can be detected/identified easily and rapidly.

主权项

1. A method of detecting Plasmodium falciparum in a biological sample with a malarial infection, the method comprising:
a) extracting DNA from the biological sample; b) amplifying a region of a Plasmodium 18S rRNA gene sequence by reacting the DNA extracted in step (a) in a reaction mixture containing a strand displacement DNA polymerase and a sequence-specific primer set which is an oligonucleotide set containing nucleic acid sequences represented by SEQ ID NOs: 7 to 12; and c) detecting the presence or absence of an amplified product of step (b); wherein the presence of said amplification product is indicative of the presence of Plasmodium falciparum in the biological sample.