| 主权项 |
1. A method of performing a measurement with a sensor having a sensing surface and at least one capture molecule attached to the sensing surface for forming a binding pair with an analyte of interest, the binding pair having a flexible spatial orientation, the method comprising:
capturing the analyte of interest with the capture molecule, thereby forming the binding pair; performing a sensor measurement with the binding pair in an initial spatial orientation; applying a first electromagnetic force to the sensing surface to alter the spatial orientation of the binding pair, wherein the magnitude of the applied first electromagnetic force is less than the binding energy of the binding pair; performing a sensor measurement with the binding pair in the altered spatial orientation; deriving a sensor signal induced by the binding pair from the sensor measurement with the binding pair in the initial spatial orientation and the sensor measurement with the binding pair in the altered spatial orientation, wherein deriving the sensor signal induced by the binding pair comprises calculating a difference between the sensor measurement with the binding pair in the initial spatial orientation and the sensor measurement with the binding pair in the altered spatial orientation; and repeating, at a predefined frequency, the steps of performing a sensor measurement with the binding pair in the initial spatial orientation, applying the first electromagnetic force, performing the sensor measurement with the binding pair in the altered spatial orientation, and deriving a sensor signal. |
