| 摘要 |
A method for using a thermostable phytase for eliminating or reducing phytic acid or salts of phytic acid in an alcohol production process is disclosed. The phytase can be added anywhere in the alcohol production process including a feedstock, a hammer mill, a slurry tank, a jet cooker, a liquefaction, a mash cooker, a fermentation, a beer, a distillation system, a whole stillage, a centrifuge, a thin stillage, an evaporator, a condensate, a syrup, a wet grain, a drum dryer, a distillers dried grain, distillers solubles, distillers wet grain, condensed distillers solubles distillers dried grains with solubles a molecular sieves, or any combination thereof. The alcohol production process can be in an ethanol production plant; a spirit or a drinkable alcohol production plant; or a fuel ethanol plant. |
| 主权项 |
1. A method for eliminating or reducing phytic acid and salts of phytic acid in an alcohol production process comprising:
(a) providing a variant polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 2 and one single amino acid substitution to the amino acid sequence of SEQ ID NO: 2, wherein said one single amino acid substitution is selected from the group consisting of: A47F, T136F, N159V, N159E, T163R, D164R, G179R, V233W, Q275V, R289A, and T349Y; wherein said variant polypeptide has phytase activity and; (b) providing a composition comprising a phytic acid or a salt of phytic acid; and (c) contacting said variant polypeptide of (a) with said composition of (b), wherein said variant polypeptide hydrolyzes the phytic acid or salt of phytic acid. |
