Cleavage sensitive antibodies and methods of use thereof

摘要

We disclose cleavage-sensitive antibodies with epitopes spanning the scissile bond of the toxins molecular target protein, enabling toxin-associated proteolysis to be measured in a variety of assay formats.

主权项

1. A proteolytic toxin assay comprising the steps of:
(a) combining a test compound with a substrate and antibody, either sequentially or simultaneously, wherein the substrate has a cleavage site for a toxin and when cleaved by the toxin forms a product, wherein the antibody binds to the substrate but not to the product, wherein the substrate is within a cell; and, (b) detecting the presence of antibody bound to the substrate;wherein said antibody binds selectively to a peptide having a sequence selected from the group consisting of:(SEQ ID NO: 1)RIDEANQRATKMLGSG;(SEQ ID NO: 2)DEANQRATKMLGSG;and,(SEQ ID NO: 3)RIDEANQRATKMLG.