Compositions, methods and uses

摘要

The present invention relates to a class of engineered polypeptides having a binding affinity for albumin. It also relates to new methods and uses that exploit binding by these and other compounds to albumin in different contexts, some of which have significance for the treatment of disease in mammals including humans.

主权项

1. An engineered albumin binding polypeptide comprising an albumin binding motif, which motif consists of the amino acid sequence:GVSDX5YKX8X9I X11X12AX14TVEGVX20 ALX23X24X25I(SEQ ID NO: 516)wherein, independently of each other,
X5 is selected from Y and F; X8 is selected from N, R and S; X9 is selected from V, I, L, M, F and Y; X11 is selected from N, S, E and D; X12 is selected from R, K and N; X14 is selected from K and R; X20 is selected from D, N, Q, E, H, S, R and K; X23 is selected from K, I and T; X24 is selected from A, S, T, G, H, L and D; and X25 is selected from H, E and D;with the proviso that the amino acid sequence is notGVSDYYKNLI NNAKTVEGVK ALIDEI; or(SEQ ID NO: 517)GVSDYYKNLI NNAKTVEGVN ALKAEI(SEQ ID NO: 541)the albumin binding polypeptide binding to albumin such that the KD value of the interaction is at most 1×10−9 M.