Nucleic acid amplification using microfluidic devices

摘要

The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certain devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyzes, including various primer extension reactions and nucleic acid amplification reactions.

主权项

1. A microfluidic peristaltic pump comprising a microfluidic flow channel and a first valve, a second valve, and a third valve, wherein the valves occur in a consecutive order along a length of the flow channel and wherein the valves are operably linked such that a single actuation force closes the valves in sequence thereby creating a pumping action through the channel, wherein the sequence is: the first valve is closed before the second valve is dosed, and the second valve is closed before the third valve is dosed.