Decellularized omentum matrix and uses thereof

摘要

Methods for defatting omentum and processes for preparing an acellular omentum, i.e., devitalized or decellularized omentum, comprising extracellular matrix for implantation into a mammalian system. Constructs for medical applications comprising decellularized omentum are also described. More specifically, mesh reinforced omentum biomatrix for soft tissue repair is described.

主权项

1. A method of delipidizing an omentum, the method comprising:
(i) freeze-drying an omentum; (ii) heating the freeze-dried omentum to a temperature in the range of about 37° C. to about 40° C.; (iii) compressing the heated omentum to express fats and other lipids, thereby yielding a partially-delipidized omentum, wherein the step of compressing the heated omentum comprises gradually increasing the force of compression to at least about 22,000 lbs.; and (iv) contacting the partially-delipidized omentum with an extraction solvent to extract therefrom at least some of the remaining fats and other lipids, wherein (A) the extraction solvent is selected from the group consisting of hexane, cyclohexane, xylene, benzene, toluene, ethyl acetate, acetone, dioxane, acetonitrile, and combinations thereof, or (B) the extraction solvent is supercritical carbon dioxide.