Neural transfection reagents

摘要

The invention is directed to transfection reagents for the delivery of nucleic acids into neural cells, compositions including the reagents, methods of preparation of such reagents, methods of transfecting cells with such reagents, and uses thereof. In preferred embodiments the reagents comprise horseradish peroxidase and/or a polycarboxylic acid such as poly(acrylic acid) or poly(methacrylic acid).

主权项

1. A transfection reagent comprising
(a) a neural transport agent comprising an enzyme, polymer, lectin, fluorescent dye, dextran, or a combination of any two or more of these, and (b) a nucleic acid carrier disposed within and conjugated to the neural transport agent, wherein the nucleic acid carrier and the neural transport agent comprise complementary chemically reactive groups or high affinity ligands to conjugate the carrier and the transport agent, and wherein the transfection reagent has a net negative surface charge under mammalian physiological conditions and an overall effective diameter of 5 to 250 nm.