| 发明名称 |
Humanization of rabbit antibodies using a universal antibody framework |
| 摘要 |
The present invention relates to an universal antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a universal antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications. |
| 申请公布号 |
US8937162(B2) |
申请公布日期 |
2015.01.20 |
| 申请号 |
US201213616214 |
申请日期 |
2012.09.14 |
| 申请人 |
Esbatech, An Alcon Biomedical Research Unit, LLC |
发明人 |
Borras Leonardo;Urech David |
| 分类号 |
C07K16/18;C07K16/22;C07K16/24 |
主分类号 |
C07K16/18 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of providing a human variable heavy chain acceptor framework for the grafting of lagomorph heavy chain CDRs, comprising changing the amino acid at one or more of positions 24, 56, 84, 89 and 108 (AHo numbering) of a human variable heavy chain framework sequence so that the resulting framework sequence comprises at least four of the following amino acids: threonine (T) at position 24, alanine (A) or glycine (G) at position 56, threonine (T) at position 84, valine (V) at position 89, and arginine (R) at position 108 (AHo numbering), whereby the resulting human variable heavy chain acceptor framework is an acceptor for the heavy chain CDRs from a donor lagomorph immunobinder. |
| 地址 |
CH |
