Un metodo de deteccion homogeneo para un proceso de pcr usando fret que detecta la presencia o ausencia de un snp de haahasll-a122(at)t dentro del gen haahasll.

摘要

A method consisting of a homogeneous assay detection system for a PCR process using FRET for detection and zygosity analysis of the HaAHASL1-A122(At)T single nucleotide polymorphism in sunflower is provided. The method provides specific sunflower-genome primers that can be used to detect the presence or absence of the HaAHASL1-A122(At)T single nucleotide polymorphism. The primer combinations for use in an endpoint PCR assay capable of determining zygosity and for assisting in breeding introgression are described.