| 摘要 |
Methods, devices, and compositions described herein are directed to artificially evolving an organism for use as a biocontrol agent. Methods, devices, and compositions described herein are useful for evolving a microorganism to acquire traits not naturally associated with the microorganism. The artificial evolution process can utilize culture methods and devices designed to accommodate particular culture methods described herein. The organism can be artificially evolved for a characteristic such as ultraviolet light tolerance, chemical tolerance, thermotolerance, enhanced growth rate on a target carbon source, host specific growth, modified sporulation characteristics or modified spores. |
| 主权项 |
1. A method of controlling a pest comprising:
(a) applying an artificially evolved microorganism to an area infected by a pest, wherein said artificially evolved microorganism was artificially evolved by: continuously culturing a parent microorganism with said pest, an extract of said pest, a component of said pest, or a combination thereof, for sufficient time so that said artificially evolved microorganism has one or more traits that increase said artificially evolved microorganism's ability to inhibit said pest compared to said parent microorganism; wherein said one or more traits comprise enhanced tolerance to a chemical, enhanced growth rate on a target carbon and/or nitrogen source, modified sporulation characteristics, increased production of a naturally produced enzyme, enhanced growth rate on said pest, an extract of said pest, a component of said pest, or a combination thereof; and (b) inhibiting said pest with said artificially evolved microorganism. |
