发明名称 |
Recombinant enzyme for converting maltose into trehalose |
摘要 |
A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose. |
申请公布号 |
EP0695804(A3) |
申请公布日期 |
1997.01.29 |
申请号 |
EP19950303933 |
申请日期 |
1995.06.08 |
申请人 |
KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJO |
发明人 |
KUBOTA, MICHIO;TSUSAKI, KEIJI;SUGIMOTO, TOSHIYUKI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/90;C12N15/61;C12P19/24;C12R1/01 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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