发明名称 |
METHOD FOR DETECTING AND CONTROLLING SUPPLY OF AN IMMERSION MEDIUM |
摘要 |
A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process. |
申请公布号 |
US2015015942(A1) |
申请公布日期 |
2015.01.15 |
申请号 |
US201414319246 |
申请日期 |
2014.06.30 |
申请人 |
Carl Zeiss Microscopy GmbH |
发明人 |
Steinmeyer Ralf;Mitzkus Reiner;Rojahn Timo |
分类号 |
G02B21/24;G02B21/33 |
主分类号 |
G02B21/24 |
代理机构 |
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代理人 |
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主权项 |
1. A method for detecting and controlling a supply of an immersion medium into an immersion film region disposed between a front lens of a microscope objective of a microscope having an autofocus device and a preparation being examined with the microscope, by using a light source reflected into a microscope beam path of the microscope with a beam splitter, the method including:
recording and evaluating a state of the immersion film region with a detection unit of the microscope or a detection unit of the autofocus device, using an autofocus signal of the light source or a reflection of a modulation object arranged in an intermediate image plane of an incident light illumination source; communicating the state of the immersion film region to a control unit; and using the control unit, changing a characteristic of the supply of the immersion medium based on the state of the immersion film region. |
地址 |
Jena DE |