Side-chain protected oligopeptide fragment condensation using subtilisins in organic solvents

摘要

Method for enzymatically synthesising an oligopeptide, comprising the coupling of an (optionally N-protected) protected oligopeptide ester with an (optionally C-protected) protected oligopeptide nucleophile in an organic solvent or an organic solvent mixture having a water content of 0.1 vol% or less, by a subtilisin in any possible form.