发明名称 |
METHOD AND KIT FOR DETECTING 5-HYDROXYMETHYLCYTOSINE IN NUCLEIC ACIDS |
摘要 |
Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample. |
申请公布号 |
EP2698437(A4) |
申请公布日期 |
2015.01.14 |
申请号 |
EP20120771081 |
申请日期 |
2012.04.16 |
申请人 |
RIKEN |
发明人 |
OKAMOTO, AKIMITSU;SUGIZAKI, KAORI;NAKAMURA, AKIKO;YANAGISAWA, HIROYUKI;IKEDA, SHUJI |
分类号 |
C12Q1/68;C12N15/09 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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