Method for selecting reduced differentiation resistance human induced pluripotent stem cells

摘要

The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.

主权项

1. A method for selecting human induced pluripotent stem (iPS) cells having reduced differentiation resistance, said method comprising the steps of: (1) inducing differentiation of human iPS cells in said population;
(2) detecting remaining undifferentiated cells after the step (1) and determining the percentage of said undifferentiated cells in said population, wherein the undifferentiated cells are detected by measurement of undifferentiated cell marker selected from the group consisting of S SEAA-3, S SEA-4, TRA-1-60, TRA-1-81, OCT3/4, Nanog, and Rex 1; and (3) (i) determining said population as having reduced differentiation resistance when the percentage of remaining undifferentiated cells detected in step (2) is equivalent to or not more than the percentage of remaining undifferentiated cells after induction of differentiation in reduced differentiation resistant human embryonic stem cells H9, KhES1 or KhES3, or (ii) determining said population as having reduced differentiation resistance when the percentage of remaining undifferentiated cells detected in step (2) is not more than 4.7%.