发明名称 |
Process and system for obtaining botulinum neurotoxin |
摘要 |
Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use. |
申请公布号 |
US8932827(B2) |
申请公布日期 |
2015.01.13 |
申请号 |
US201213407662 |
申请日期 |
2012.02.28 |
申请人 |
Allergan, Inc. |
发明人 |
Ton Jennifer L.;Patel Hemant A.;Bates Ronald C.;Ahmad Wajdie M. |
分类号 |
C12P1/00;C12P21/04;C12N9/52;C07K14/33 |
主分类号 |
C12P1/00 |
代理机构 |
|
代理人 |
Phan Brigitte C.;Chan Ted A.;Condino Debra |
主权项 |
1. A substantially animal product free (APF) process utilizing chromatography for obtaining a botulinum neurotoxin, the process comprising the following steps:
(a) providing a substantially APF fermentation medium; (b) fermenting clostridium botulinum bacteria in the fermentation medium, wherein the clostridium botulinum bacteria produces a botulinum neurotoxin, and; (c) recovering the botulinum neurotoxin from the fermentation medium by contacting the fermentation medium with an anion exchange chromatography media followed by contacting a first eluate from the anion exchange chromatography medium with a cation exchange chromatography medium, followed by contacting a second eluate from the cation exchange chromatography medium with a hydrophobic interaction chromatography medium, eluting a third eluate from the hydrophobic interaction chromatography medium, thereby obtaining the botulinum neurotoxin from the substantially APF chromatography process. |
地址 |
Irvine CA US |