Analyte mass spectrometry quantitation using a universal reporter

摘要

Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.

主权项

1. A method of generating a dilution curve in a single injection into a liquid chromatograph/mass spectrometer, the method comprising
injecting into a first liquid chromatography (LC) column coupled to a mass spectroscopy detection system a single peptide composition, each peptide in the composition having the identical amino acid sequence A whereby each amino acid is present in the composition as a heavy labeled isomer resulting in the composition being a sequence A blend of all heavy labeled isomers, each isomer in the blend of sequence A having a mass shift and each isomer in the blend of sequence A present in a different concentration, analyzing each peptide in the co-eluted peptide composition by mass spectroscopy, and generating from the single peptide composition injection of sequence A into the LC column a dilution curve from the analysis.