Lantibiotics and uses thereof

摘要

The present invention provides isolated lantibiotics that inhibit. Gram negative and Gram positive microbes. The antibiotic includes an amino acid sequence, wherein the amino acid sequence of the compound and the amino acid sequence of SEQ ID NO:21 or SEQ ID NO:22 have at least 80% identity. The lantibiotics have the characteristic of inhibiting growth of a Gram negative microbe in conditions that do not damage the outer membrane of the Gram negative microbe. The present invention also provides methods for making and using the lantibiotics.

主权项

1. A composition comprising a lantibiotic and a food product, wherein the lantibiotic is not inactivated by digestion with trypsin at 25° C. for 24 hours, wherein the amino acid sequence of the compound and the amino acid sequence of SEQ ID NO:21 have at least 80% identity, and wherein SEQ ID NO:21 is proline-alanine-Xaa1-leucine-Xaa2-phenylalanine-alanine-valine-Xaa3-valine-leucine-Xaa4-valine-Xaa5-phenylalanine-Xaa6-alanine-Xaa7-Xaa8-valine-Xaa9-valine-valine-Xaa10-arginine-leucine-alanine-Xaa11-Xaa12-glycine-asparagine-Xaa13-lysine, where Xaa1, 2, 3, 4, 5, 6, 8, and 11 are each independently serine, didehydroalanine, or alanine, Xaa7, 12, and 13 are each independently cysteine or alanine, and Xaa9 and 10 are each independently threonine, didehydrobutyrine, or 2-aminobutyric acid.