Device for preparation of liposomes and method thereof

摘要

Disclosed is a device for preparation of liposomes, comprises a reaction tank and an infusion unit. The reaction tank comprises a collector mounted in a predetermined position of the reaction tank; Two inlet ports are included: the first inlet port for infusing an aqueous solution; and the second inlet port for infusing an organic solution. The infusion unit can introduce a bioactive agent containing-aqueous solution into the reaction tank. The infusion unit comprises a filter connected to one end of the infusion unit and being adjacent to the collector. The method using the device comprises the steps of infusing an aqueous solution and an organic solution into the reaction tank of the device and thus forming an interface between the filter and the collector; infusing a bioactive agent containing-aqueous solution and being filtered by the filter, the bioactive agent is encapsulated to form a water-in-oil emulsion; the water-in-oil emulsion is passing through the aqueous solution and thus to form a water-in-oil-in-water double emulsion. Finally the removal of the organic phase of water-in-oil-in-water double emulsion enables the harvest a plurality of liposomes. It has advantages such that simple-used and automation production. Thus nano size or sub-micro size liposomes can be prepared with high encapsulation efficiency without sonicators or delicate microfluidic systems.

主权项

1. A device for preparation of liposomes, which is programmable and adapted to manufacture liposomes, comprising:
a reaction tank, comprising:
a collector mounted in an upper portion of the reaction tank;a first inlet port set at the upper portion of the reaction tank for infusing an aqueous solution; anda second inlet port set at a lower portion of the reaction tank for infusing an organic solution; an infusion unit set at the lower portion of the reaction tank and being opposite to the collector, wherein the infusion unit has a first end in the reaction tank and a second end out of the reaction tank for introducing a bioactive agent containing-aqueous solution into the reaction tank; and a filter deposited at the first end in the reaction tank of the infusion unit and being adjacent to but separated from the collector.