Vault and vault-like carrier molecules

摘要

A method of using vaults as carrier molecules to deliver one or more than one substance to an organism, or to a specific tissue or to specific cells, or to an environmental medium. A vault-like particle. A method of preventing damage by one or more than one substance to an organism, to a specific tissue, to specific cells, or to an environmental medium, by sequestering the one or more than one substance within a vault-like particle. A method of delivering one or more than one substance or a sensor to an organism, to a specific tissue, to specific cells, or to an environmental medium. According to another embodiment of the present invention, there is provided a method of making vault-like particles, and making vault-like particles comprising one or more than one substance, or one or more than one sensor.

主权项

1. An assembled vault-like particle having a substance to be delivered comprising multiple copies of a modified naturally occurring sequence of MVP, where the naturally occurring sequence of MVP is modified by adding an amino acid sequence to the N-terminal of the naturally occurring sequence of MVP which results in one or more than one substance-binding domain within the assembled vault-like particle, and the substance to be delivered is selected from the group consisting of an enzyme, a pharmaceutical agent, a plasmid, a polynucleotide, a polypeptide, a sensor, and a combination of the preceding.