System and device for characterizing cells

摘要

A diagnostic device includes a microscope configured to obtain image data on a plurality of cells and a computing device. The computing device is configured to receive the image data, identify at least a portion of each of the plurality of cells based on the received image data, determine at least one of a value of a morphological parameter for each identified at least a portion of the plurality of cells or a relative organization among the identified at least a portion of the plurality of cells, and calculate statistics for the plurality of cells based on the at least one of the determined values of the morphological parameter or the determined relative organization, the statistics including information suitable for distinguishing metastatic cells from non-metastatic cells. The diagnostic device further includes an output device configured to output the statistics for diagnosis.

主权项

1. A diagnostic device comprising:
a microscope configured to obtain image data on a plurality of cells; a computing device configured to:
receive the image data;identify at least a portion of each of the plurality of cells based on the received image data;determine at least one of a value of a morphological parameter for each identified at least a portion of the plurality of cells or a relative organization among the identified at least a portion of the plurality of cells; andcalculate statistics for the plurality of cells based on the at least one of the determined values of the morphological parameter or the determined relative organization, the statistics including information suitable for distinguishing metastatic cells from non-metastatic cells; and an output device configured to output the statistics for diagnosis, wherein said identifying the at least a portion of each of the plurality of cells comprises:
normalizing the image data;segmenting nuclei of the plurality of cells in the normalized image data; andidentifying the at least a portion of each of the of the plurality of cells based on the segmented nuclei, and wherein said segmenting nuclei comprises:
obtaining Gaussian intensity image data of the normalized image data;obtaining averaged intensity image data of the normalized image data;obtaining nuclear intensity image data based on the difference between the Gaussian intensity image data and the averaged intensity image data; andsegmenting nuclei based on the nuclear intensity image data.