Methods for controlling pests using RNAi

摘要

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for producing transgenic cells expressing the double stranded RNA molecules, as well as compositions and commodity products containing or treated with such molecules.

主权项

1. A method for controlling insect infestation, comprising exposing an insect to a composition comprising a double stranded ribonucleotide that inhibits the expression of an mRNA transcribed from a polynucleotide sequence selected from the group consisting of
(i) a polynucleotide sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 11; (ii) a polynucleotide sequence comprising a nucleic acid sequence having at least 70% sequence identity over a comparison window of at least 100 nucleotides as determined by the BLASTN alignment tool to the nucleic acid sequence set forth in SEQ ID NO: 11; and (iii) a polynucleotide sequence comprising a fragment of at least 21 nucleotides of SEQ ID NO: 11, and wherein the double stranded RNA is produced by a bacterium or yeast cell engineered to produce the double stranded RNA from a DNA construct comprising annealed complementary strands, one of which has a polynucleotide selected from any of (i)-(iii), or wherein the double stranded RNA is in vitro transcribed from a DNA construct comprising annealed complementary strands, one of which has a polynucleotide sequence selected from the any of (i)-(iii).