| 摘要 |
FIELD: agriculture.SUBSTANCE: invention relates to the field of agriculture, in particular to selection. The invention is a method of increasing the coefficient of white cabbage reproduction under conditions in vitro, comprising growing the explants, cultivation of them in a nutrient medium of Murashige and Skoog, applying to it of growth regulators thidiazuron at a concentration of 1 mg/l in combination with the indolyl-3-acetic acid - 0.5 mg/l, using receptacles with the size of 0.2-0.3 mm, isolated from buds with the length of 0.5-0.7 mm, where the receptacle is used 1-2 days before flower blooming, and after growing them in nutrient media it is cultured till buds formation for 14-21 days.EFFECT: method enables to obtain greater amount of regenerated plants with signs of CMS and to obtain genetically stable crop of selective samples.3 tbl |
