METHOD AND ARRANGEMENT FOR RECORDING MOLECULAR BINDING PHENOMENA

摘要

Method and arrangement for recording molecular binding phenomena, where according to the invention at least one second molecule is or has been bound, with formation of at least one specific non-covalent bond, to at least one first molecule which has been immobilized on a bioactive surface by a covalent bond, the second molecule here being or having been bound covalently to a ligand. The bioactive surface kann be brought into contact with at least one analyte molecule, and binding phenomena between the analyte molecule and the ligand can be recorded here by use of an analytical test method. The at least one specific non-covalent bond between the first and second molecule can in turn be broken by introducing a buffer solution. A third molecule can optionally be bound to the second molecule, and the third molecule here can also have a ligand. This is an easily reversible method for providing, on the bioactive surface, a particular ligand or two particular ligands that differ from one another.