MEMBRANE SPAN-KINASE FUSION PROTEIN AND THE USES THEREOF

摘要

The disclosure relates to a recombinant membrane span protein complex, comprising (1) a fusion protein, comprising a membrane span protein fused to a kinase domain, preferably a constitutive kinase and (2) a reporter construct comprising a polypeptide, interacting with the membrane span protein, fused to a reporter phosphorylation domain. The disclosure relates further to the uses of such membrane span protein complex for the detection of compounds that interact with the membrane span protein and for the screening and/or detection of inhibitors of the compound-membrane span protein interactions. In a preferred embodiment, the membrane span protein is a G protein coupled receptor (GPCR) and the method is used for the screening and/or detection of inhibitors of the ligand-receptor binding.

主权项

1. A recombinant membrane span protein complex comprising:
a first fusion protein comprising a membrane span protein chain fused to either a kinase domain or a reporter phosphorylation domain, and a second fusion protein comprising a polypeptide, interacting with the membrane span protein chain, fused to either a reporter phosphorylation domain or a kinase domain, complementary to the first fusion protein.