PNEUMATICALLY/HYDRAULICALLY ACTUATED FLUOROPOLYMER-HPLC CHROMATOGRAPHIC SYSTEM FOR USE WITH HARSH REAGENTS

摘要

The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer. The system comprises at least one reagent reservoir; at least one mixing chamber, wherein the contents of the at least one reagent reservoir are combined; at least one pump that transfers the contents of the at least one reservoir to the mixing chamber; and at least one modular elution column, wherein the at least one modular elution column contains a temperature control means; a sample injection system connected to an injection loop or 3-way valve to inject the sample solutions in the modular elution columns, at least one manifold or X-Y moving stage to distribute the eluted volumes in different receptacles; at least one return line to automatically reinject selected elution fractions at the top of the column; wherein all moving components of the said system are fluid actuated.

主权项

1. A high-performance liquid chromatography system wherein all moving components of that said system are actuated by fluid pressure, said system comprising:
a. at least one reagent reservoir, wherein the at least one reservoir contains a temperature control means; b. a mixing chamber, wherein the contents of the at least one reagent reservoir are combined, and wherein the mixing chamber contains a temperature control means; c. a pump that transfers the contents of the at least one reservoir to the mixing chamber; d. a modular elution column, wherein the column contains a temperature control means; e. a means for injecting one or a plurality of sample solutions in the modular elution column; f. a means for distributing eluted volumes to different receptacles; wherein all moving components of the said system are pneumatically actuated and g. a return line with a pump to automatically reload the elution cut of interest onto the column.