ACRYLAMIDE-DEGRADING SELF-CLONING ASPERGILLUS ORYZAE

摘要

Provided are self-cloning Aspergillus oryzae that expresses amidase without induction culture exhibiting high amidase degradation activity, and a method for reducing acrylamide in which this self-cloning Aspergillus oryzae is used. Self-cloning Aspergillus oryzae, which has a gene which codes a polypeptide with a specific amino acid sequence indicated in SEQ ID NO:1, or has a base sequence hybridizable to a complementary sequence of the gene encoding SEQ ID No:1 under stringent conditions, has a protein with amidase activity which the gene is expressed without induction culture, the process of reducing acrylamide by contact treatment with the above described self-cloning Aspergillus oryzae and acrylamide-containing matter, and a method of producing reduced acrylamide food or beverage.

主权项

1. Self-cloning Aspergillus oryzae comprising a sequence which is hybridizable under stringent conditions with a gene that encodes a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1, or a nucleic acid molecule including a base sequence complementary to a gene that encodes the polypeptide, and a gene that encodes a protein having amidase activity introduced therein with the capability of being expressed without induction culture.