Synthetic Transcriptional Control Elements and Methods of Generating and Using Such Elements

摘要

Provided herein are nucleic acid constructs that contain a synthetic control element that includes a cis-regulator of translation, and an adapter translation-coupled regulator of transcription. Further provided herein are nucleic acid constructs that contain nucleic acid sequences under the control of the synthetic control elements. Also provided are compositions and methods related to the nucleic acid constructs.

主权项

1. A nucleic acid construct comprising a synthetic TCRT (translation-coupled regulator of transcription) comprising in a 5′ to 3′ order:
(a) a cis-regulator of translation; and (b) an adapter TCRT, wherein (a) and (b) are operably linked so that the transcription of sequences that are 3′ of and operably linked to the synthetic TCRT is regulated by (a).